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CMMG News

The Center for Molecular
Medicine and Genetics
Volume 1 Issue 1
March 1996

Wayne State University School of Medicine

Enhancing Research and Training in Molecular Medicine and Genetics
1 Greetings From the Director
2 Welcome to New Faculty
3 Graduate Training Programs
3 Activities
5 Collaborations
6 Facilities

Greetings From the Director

Robert H. Rownd, Ph.D.

The Wayne State University Board of Governors approved the university-wide Center for Molecular Medicine and Genetics (CMMG) effective Oct. 1, 1994. Its mission is to enhance research strength in molecular medicine and genetics at WSU and to develop interdisciplinary research and training programs in targeted human health areas that bridge basic and clinical science. By promoting interactions and collaborations throughout the university and its affiliated hospitals, CMMG programs should provide new opportunities for external funding and foster translational research and technology transfer.

The CMMG currently has 19 primary, 9 joint, and 6 associate/adjunct faculty members. The majority of the CMMG faculty also have appointments and close associations with clinical departments. In addition to their individual research programs, the CMMG faculty are developing interdisciplinary programs with clinical scientists in areas such as human genetics and gene therapy, cancer, AIDS, neurological diseases and human reproductive biology. These interactions and collaborations include faculty in the WSU departments of Internal Medicine, Neurology, Pathology, Obstetrics and Gynecology, Surgery, Pediatrics, Emergency Medicine, Urology, and Psychiatry and Behavioral Neurosciences. Joint publications and research grant applications have resulted from some of these collaborative efforts.

The CMMG is broadening its academic programs to make them more interdisciplinary and to provide new opportunities for medical, graduate and postdoctoral student research in more clinically oriented areas. The number of faculty participating in the existing molecular biology and genetics graduate program has more than doubled in the last two years. More graduate students are carrying out their doctoral research in clinical departments. A proposal for a new M.S. degree program in genetic counseling has been submitted for approval and a program director has been hired. Other new graduate programs are being developed. Focused training programs and research opportunities for medical students, residents, fellows and clinical faculty will be offered, including courses and workshops on techniques, methodologies and research approaches in molecular medicine and genetics.

An important CMMG goal is to promote communication and interaction with medical biotechnology companies to facilitate the application of discovery research to the prevention, diagnosis and treatment of diseases, and to promote economic development in the state of Michigan.

The CMMG is providing research training opportunities for residents, fellows and faculty from several clinical departments and intends to expand these opportunities as the center continues to grow and develop. It has a Macromolecular Core Facility for nucleic acid synthesis and sequencing, and a shared Biocomputing Facility that have been used extensively by faculty and students in more than a dozen departments, centers, and institutes. CMMG faculty and staff are also available for advice and strategy in the design and interpretation of experiments involving molecular and cellular approaches.

"Faculty interested in a CMMG appointment should send an application letter and curriculum vitae to the director at 3216 Scott Hall ..."

Many of the CMMG goals and programs are prospective in nature since the center is in the early stages of its development. The implementation and achievement of these future objectives will be greatly facilitated by the involvement and participation of faculty and students throughout the university and its affiliated hospitals, who are strongly encouraged to become involved with center programs and activities. Faculty interested in a CMMG appointment should send an application letter and curriculum vitae to the director at 3216 Scott Hall (telephone 313-577-5323).

CMMG Welcomes 13 New Faculty Members

John Kamholz, M.D./Ph.D., joined the CMMG as a joint associate professor with tenure in the WSU Department of Neurology in the summer of 1994. Dr. Kamholz had been an Assistant Professor at the University of Pennsylvania School of Medicine, where he initiated studies on human myelin basic protein gene regulation. Dr. Kamholz's recruitment provided a bridge between the department of neurology and the CMMG that has been strengthened with the recent recruitment of Dr. James Garbern.

John Tomkiel, Ph.D., joined the CMMG in November of 1994 as an assistant professor from the Johns Hopkins University School of Medicine where he completed a postdoctoral fellowship. His tenure-retreat department is biological sciences. He received his Ph.D. in genetics in 1990 from the University of Washington. Dr. Tomkiel’s research focus is the function of the centromere in cell division and its aberration in cancer and birth defects.

Anne Greb, M.S., became an assistant professor in the CMMG in January of 1995 on the clinical-educator track. She is the director of the Genetic Counseling Graduate Program that is currently being reviewed for approval as a new master's program. Professor Greb trained at the University of Wisconsin, Madison, and has been a staff genetic counselor since 1986, with six of those years at Hutzel Hospital, where she participated in the medical teaching program of the Reproductive Genetics Unit.

Robert A. Thomas, Ph.D., was promoted in May 1995 to assistant professor (research) after completing a postdoctoral fellowship in the CMMG. He received his Ph.D. from the department of biological sciences at WSU in 1992. Dr. Thomas's research using HIV-1 to study the infection and transcriptional activation of retroviruses in human fetal development in collaboration with Drs. A. Scott Goustin (CMMG), Theodore Jones (Obstetrics-Gynecology) and James Jarvis (Pediatrics) will be extended to include HIV-2.

S. Helena Kuivaniemi, M.D./Ph.D., joined the CMMG in September of 1995 as a tenure-track associate professor with tenure-retreat in the department of surgery. She came from Jefferson Medical College at Thomas Jefferson University in Philadelphia where she worked on the molecular basis of a number of connective tissue disorders and, more recently, of aortic aneurysms. Her studies in that area focused on the candidate gene approach and led to the identification of mutations in the gene for type III procollagen that were associated with aortic aneurysms in some families. At WSU, Dr. Kuivaniemi, in collaboration with Professor Anne Greb, and Drs. Ramon Berguer, Andris Kazmers and Ronald Kline (surgery), will continue to collect familial cases of abdominal aortic aneurysms in order to use DNA linkage analysis to identify the gene(s) involved in the development of aneurysms. Her work will also include differential display PCR to identify unique genes expressed in the aorta.

Gerardus Tromp, Ph.D., joined the CMMG as an assistant professor (research) in September, 1995. He came from Thomas Jefferson University in Philadelphia, where he was a research assistant professor. Dr. Tromp received his Ph.D. in 1989 from Rutgers University and completed his postdoctoral training at Jefferson Medical College. His research involved defining the molecular basis of several connective tissue disorders such as osteogenesis imperfecta and variants of the Ehlers Danlos syndrome. Recently, he has worked on intracranial aneurysms, attempting to define the gene(s) predisposing individuals to the disease, initially by using the candidate gene approach and subsequently by genetic epidemiology and linkage. At WSU, in collaboration with Dr. Antti Ronkainen (currently at WSU), members of the neurosurgery department, and the University of Kuopio, Finland, he will continue to collect information on families with intracranial aneurysms to define the gene or genes that predispose the disease by using linkage analyses. Furthermore, he hopes to establish other collaborative projects with WSU faculty involving genetic epidemiology and linkage.

Russell L. Finley, Jr., Ph.D., came from the Harvard Medical School Department of Genetics in September 1995 as an assistant professor in the CMMG with tenure-retreat in the department of internal medicine, hematology/oncology division. He had trained at the Massachusetts General Hospital following his Ph.D. training (1990) at State University of New York, Syracuse. Dr. Finley will continue his research on understanding the regulatory networks that control cell proliferation during development, employing a powerful genetic interaction trap methodology that he helped develop, along with conventional molecular and genetic techniques.

James Garbern, M.D./Ph.D., became a joint assistant professor in the CMMG and neurology department in September 1995. He came from the University of Pennsylvania School of Medicine where he had been an assistant professor studying the molecular genetics of neurological disease. Dr. Garbern carried out his medical, doctoral and postdoctoral training at Baylor College of Medicine and the National Institutes of Health. At WSU, his research focus is the cloning and analysis of human genes involved in the regulation of myelination. Dr. Garbern is currently organizing a Neurogenetics Center at WSU with Professor Greb and Dr. Kamholz.

In addition to the new primary faculty, we welcome the following new associate faculty members: James Jarvis, M.D. (pediatrics), Donald Kuhn, Ph.D. (psychiatry and behavioral neurosciences), Kenneth Maiese, M.D. (neurology), Adnan Munkarah, M.D. (obstetrics and gynecology), and Michael Shy, M.D. (neurology).

CMMG Graduate Training Programs

CMMG currently offers a graduate program in molecular biology and genetics (MBG) with Ph.D. and master's degrees. Currently 25 students are enrolled in the Ph.D. program, two of whom are M.D.-Ph.D. candidates, and two master's candidates. In the past year CMMG has awarded 7 Ph.D. degrees and two master's degrees. Our congratulations go to Drs. Scott Smith, VijayaLaksmi Shridhar, Kristin Gutridge, Shiping Tang, William Paradee, Timothy Drumheller, and Chris Krebs, who have gone on to promising postdoctoral positions. We wish them all the best in their careers.

We offer further congratulations for recent MBG Ph.D. recipient Chris Krebs, who won the Duncan McCarthy Award for the best comprehensive abstract submitted by a student or postdoc to the annual (1995) meeting of the Michigan Chapter of Neurosciences.

"The CMMG is broadening its academic programs to make them more interdisciplinary and to provide new opportunities ..."

The CMMG has also formulated a Genetic Counseling Graduate Program (with Prof. Anne Greb as director) that is currently under review by the Graduate School. The objective of the program is to provide students with the skills to function as productive and responsible genetic counselors in a variety of work settings. Students will have the opportunity to interact with numerous clinical genetic professionals including genetic counselors, pediatric and obstetrical medical geneticists, cytogeneticists, and biochemical and molecular geneticists. The program is designed to meet the criteria for accreditation by the American Board of Genetic Counseling, and will therefore integrate scientific knowledge and counseling skills with different clinical experiences. The course work consists of: principles of human and medical genetics, molecular biology, and counseling and interviewing skills. Students will obtain supervised clinical experience in a variety of genetics and subspecialty clinics, as well as in genetic service laboratories throughout The Detroit Medical Center and surrounding hospitals. Pending its approval as a new graduate program, it will admit the first class of four students for the fall of 1996.


CMMG Activities

CMMG is a Clinical Genetics Resource

The Center for Molecular Medicine and Genetics is available to assist health care providers in gathering the following information:

1. Availability of genetic testing for specific diseases;
2. Disease-specific patient support groups;
3. Statewide clinical genetic services and specialty clinics.

The CMMG is also committed to continuing education for health care providers and those in training regarding the implications of genetic testing, and is available for inservices and other educational programs. For more information please contact Professor Anne Greb at 313-577-6298 or agreb@cmb.biosci.wayne.edu

CMMG Faculty and Their Research

(For new faculty, see the article on page 2.)

Leon Carlock, associate professor (also with anatomy and cell biology); Ph.D., Purdue, 1981. Molecular neurobiology; Huntington's disease; neuron-specific gene expression in the caudate nucleus.

Mark I. Evans, Professor (also with Obstetrics and Gynecology); M.D., SUNY Downstate Medical Center, 1978. Prenatal diagnosis and fetal therapy.

Craig N. Giroux, assistant professor (also with obstetrics and gynecology); Ph.D., MIT, 1979. Cell and molecular biology of meiotic differentiation; DNA and chromosome transactions in the germ line; genetic stability and carcinogenesis; yeast biotechnology.

Morris Goodman, professor (also with anatomy and cell biology); Ph.D., Wisconsin, 1951. Molecular evolution, with emphasis on globin genes and the X-linked HPRT gene.

Anton Scott Goustin, assistant professor (research); Ph.D., California (Berkeley), 1979. Gene therapy; retroviral control of transcription; natural inhibitors of tyrosine kinases; peptide growth factor receptors in embryonic development.

Lawrence I. Grossman, associate director and professor (also with internal medicine); Ph.D., Yeshiva (Einstein), 1971. Molecular biology of mitochondria; cytochrome c oxidase genes; mitochondrial DNA.

George Grunberger, professor (also with internal medicine and director, Diabetes Program); M.D., NYU, 1977. Molecular biology of insulin action; insulin receptors; receptor gene expression in insulin resistance.

Mark Paul Johnson, associate professor (also with obstetrics and gynecology); M.D., Minnesota, 1984. Prenatal diagnosis and fetal therapy.

Minoru S. H. Ko, assistant professor (research); M.D., 1986, Ph.D., 1991, Keio (Japan). Mouse cDNA sequencing and mapping; human genome project; molecular biology of implantation; genomic imprinting.

Stephen A. Krawetz, associate professor (also with obstetrics and gynecology); Ph.D., Toronto, 1983. Gene therapy; control of development and differentiation; expression of genes controlling spermatogenesis; expression of connective tissue matrix genes in normal and pathological development; computer-assisted sequence analysis.

Gyanendra Kumar, associate professor (also with neurology); Ph.D., Lucknow (India), 1976. Molecular biology of human papovavirus JC; regulation of eukaryotic gene expression.

Markku Kurkinen, professor (also with pathology); Ph.D., Helsinki, 1979. Extracellular matrix; metalloproteinases; gene regulation; development.

Wayne Lancaster, professor (also with obstetrics and gynecology); Ph.D., Wayne State, 1973. Papillomaviruses: molecular biology, evolution, and role in human carcinogenesis.

Leonard C. Lutter, adjunct associate professor (also with Henry Ford Hospital); Ph.D., Wisconsin, 1973. Characterization of cellular and viral transcription complexes.

Dorothy A. Miller, professor (also with pathology); Ph.D., Yale, 1957. Molecular cytogenetics; mammalian centromeres; in situ hybridization; gene mapping.

Orlando J. Miller, professor (also with obstetrics and gynecology); M.D., Yale, 1950. Molecular cytogenetics; role of CpG-rich islands and DNA methylation in gene regulation; chromosome structure, genome organization, and gene amplification.

Richard E. Miller, professor (also with internal medicine and associate chief of staff for research and development, Veterans Administration Medical Center); M.D., Michigan, 1966. Study of gene expression during differentiation; transcription of insulin gene.

Jeffrey Moshier, assistant professor (also with internal medicine); Ph.D., Johns Hopkins, 1984. Molecular oncology; gene expression; role of polyamines and ornithine decarboxylase in tumorigenesis.

Mary T. Murray, assistant professor (research); Ph.D., MIT, 1987. Translational regulation of gene expression; germ line differentiation and early embryogenesis; RNA-protein interactions.

Robert H. Rownd, director and professor (also with internal medicine); Ph.D., Harvard, 1964. Gene therapy; retroviral vectors; structure, function, and replication of the genetic elements; cellular regulatory mechanisms; gene amplification.

J. Christopher States, assistant professor (research); Ph.D., Albany Medical College, Union University, 1980. Genetics of human DNA repair; bioactivation of carcinogens; gene therapy.

CMMG Research Collaborations

James Garbern, "Myelin gene expression as molecular markers for cerebral tumors," Lucia Zamorano, M.D., neurosurgery, John Kamholz, M.D./Ph.D., and Lisa Rogers, M.D., neurology.

Anton Scott Goustin, "HIV infection of developing placenta," Theodore B. Jones, M.D., obstetrics and gynocology, Fayth Yoshimura, Ph.D., and Steven R. King, Ph.D., immunology; "Natural insulin receptor tyrosine kinase inhibitor," Myron A. Leon, Ph.D., immunology, George Grunberger, M.D., and Pothur R. Srinivas, M.D., internal medicine; "Gene therapy to expand hematopoietic stem cells," Alan W. Flake, M.D., surgery, Fayth Yoshimura, Ph.D., and Steven C. Lerman, Ph.D., immunology.

Lawrence I. Grossman, "Molecular events in neuronal ischemic-reperfusion injury," Blaine C. White, M.D., and Gary Krause, M.D., emergency medicine.

George Grunberger, "Structure-function relationships of a human insulin receptor tyrosine kinase inhibitor," Myron Leon, Ph.D., immunology; "The use of insulin harmonic waveform analysis in the evaluation of pulsatile insulin secretion in patients with Type II diabetes mellitus," Michael Diamond, M.D., and Ralph Kramer, M.D., obstetrics and gynecology.

Minoru S. H. Ko, "Genetics of skin diseases," Ken Hashimoto, M.D., dermatology.

"CMMG faculty are developing interdisciplinary programs with clinical scientists in areas such as human genetics and gene therapy ..."

S. Helena Kuivaniemi, "Familial abdominal aneurysms," Ramon Berguer, M.D., Andris Kazmers, M.D., and Ronald A. Kline, M.D., surgery.

Markku Kurkinen, "Role of proteases in breast cancer progression," Rafael Fridman Ph.D., pathology; "Matrix metalloproteinases in arthritis and other degenerative diseases," Felix Fernandez-Madrid, M.D./Ph.D., rheumatology.

Wayne Lancaster, "Human papillomavirus in head and neck cancers," John Ensley, M.D., internal medicine, hematology-oncology; "Identification of tumor suppressor genes in ovarian cancer; expression of T-Cell receptor usage in cervical cancer; Role of virus in ovarian cancer," Adnan Munkarah, M.D., obstetrics-gynecology; "Association between clinical course of aids and Class II HLA," Jack Sobel, M.D. and Paula Shuman, M.D., internal medicine, infectious diseases.

Jeffrey Moshier, "Gastric mucosal injury and aging; Aging and induction of colorectal neoplasia," Adhip Majumdar, Ph.D./D.Sc., internal medicine and biochemistry; "Early weaning and gut development - Role of ornithine decarboxylase and tyrosine kinases," Chuan- Hao Lin,, M.D., pediatric gastroenterology; "The efficacy of thymosin Alpha-1 in the treatment of chronic Hepatitis B disease, Thymosin Alpha-1 bioassays utilizing Hepatitis B transfected hepatoma cells," Milton Mutchnick, M.D. and Paul Naylor, Ph.D., internal medicine.

Mary T. Murray, "Apoptosis during kidney reperfusion injury," Scott Dulchavsky, M.D., surgery, and Ken Palmer, Ph.D., pathology.

Robert H. Rownd, "Gene therapy," Lawrence Lum, M.D., formerly of internal medicine, hematology-oncology, and currently scientific director of The Adoptive Immunotherapy/Gene Therapy Program at St. Luke’s Medical Center in Milwaukee, Wis.

J. Christopher States, "Cellular and molecular toxicity of lead," Joel G. Pounds, Ph.D., institute of chemical toxicology; "Gene therapy approaches for Parkinson's disease," Donald M. Kuhn, Ph.D., psychiatry.

Robert A. Thomas, "HIV transcriptional activation," Theodore Jones, M.D., obstetrics and gynecology; "Involvement of HIV immune complexes in vertical transmission," James Jarvis, M.D., pediatrics.

CMMG Co-Sponsors AIDS Symposium

The CMMG co-sponsored the Second Conference on AIDS Research at WSU with the AIDS Research Advisory Committee, the dean of medicine, Harper Hospital, and the University of Detroit Mercy School of Dentistry. More than 100 participants attended the conference, which was held on Oct. 27, 1995 at Scott Hall. Seven WSU faculty and a faculty member from the University of Detroit Mercy School of Dentistry made oral presentations on current AIDS research. Twelve research groups presented posters on clinical and basic AIDS research.

CMMG Macromolecular Core Facility

The CMMG Macromolecular Core Facility, located in room 5177 of the Biological Sciences Building, offers two services to researchers at Wayne State University: DNA sequencing and DNA synthesis. The DNA sequencing is accomplished using an Applied Biosystems DNA Sequencer. This instrument uses four different fluorescent dyes (one for each base) to automate the base calling of the sequencing gel. Two types of fluorescence labeling are available: dye-labeled primers or dye-labeled terminators. Primers available from the facility are M13 forward and reverse, SP6, T7 and T3. Researchers provide the facility with their own primer for the second methodology. Samples can be either plasmid (single or double stranded) or PCR products. A normal gel run will produce about 400 base pairs per sample. Data files of the results suitable for analysis by computer can be provided. Each sequencing sample carries a $30 fee. The facility also has an Applied Biosystems DNA Synthesizer for synthesizing oligonucleotide primers and probes. However, this instrument has only modest production capabilities. The Center is in the process of purchasing a Beckman instrument with a significantly higher output capacity. For information about this facility, please contact Mike Hagen, Ph.D., at 313-577-1178 or mhagen@cmb.biosci.wayne.edu .

"The CMMG has a Macromolecular Core Facility and a shared Biocomputing Facility that have been used by faculty and students in more than a dozen departments, centers, and institutes..."

CMMG Shared Biocomputing Facility

The CMMG maintains a shared Biocomputing Facility for the analysis of molecular biological data. The facility runs on a Sun SPARCStation 20 that is connected to the WSU network and the Internet. The GCG Sequence Package for analysis of peptide and nucleic acid sequence data and the GenBank and EMBL databases are maintained locally. Several other tools for data analysis are also available, as is direct connectivity to the NCBI databases at NIH. The facility also provides Internet E-mail service to all faculty, students and staff in the CMMG as well as to many members of other departments in the WSU community. As part of its mission in technology transfer, the CMMG also provides Internet access and E-mail service to members of the Michigan Biotechnology Association. Currently, no charges are associated with use of the CMMG shared Biocomputing Facility. For more information, please contact David Womble, Ph.D., at 313-577-2374 or dwomble@cmb.biosci.wayne.edu

Representative Publications from CMMG Faculty

Bingham, P., Scott, M., Wang, S., McPhaul, M., Wilson, E., Garbern, J., Merry, D., and Fishbeck, K. 1995. Stability of an expanded trinucleotide repeat in the androgen receptor gene in transgenic mice. Nature Genetics 9:191-196.

Carlock, L., Walker, P.D., Shan, Y., and Gutridge, K. 1995. Transcription of the Huntington disease gene during the quinolinic acid excitotoxic cascade. NeuroReport 6:1121-1124.

Evans, M.I., Chik, L., O’Brien, J.E., Chen, B., Dvorin, E., Ayoub, M., Krivchenia, E.L., Ager, J.W., Johnson, M.P., and Sokol, R.J. 1995. MOMs and DADs: improved specificity and cost effectiveness of biochemical screening for aneuploidy with DADs. Am. J. Obstet. Gyn. 172:1138-1147.

Evans, M.I., Littman, L., St. Louis, L., LeBlanc, L., Adis, J., Johnson, M.P., and Moghissi, K.S. 1995. Evolving patterns of iatrogenic multifetal pregnancy generation: implications for aggressiveness of infertility treatments. Am. J. Obstet. Gyn. 172:1750-1753.

Greb, A.E., Sepulveda, W., Romero. R., Treadwell, M.C., Johnson, M.P., and Evans, M.I. 1995. Isolated fetal choroid plexus cyst: karyotyping is indicated. (submitted).

Gregoire L., Cubilla, A.L., Reuter, V.E., Haas, G.P., and Lancaster, W.D. 1995. Penile invasive squamous cell carcinoma: human papillomavirus preferentially associated with high grade variants. J. Nat'l. Cancer Inst. In press.

Grossman, L.I., Rosenthal, N., Akamatsu, M., and Erickson, R.P. 1995. Developmental regulation of cytochrome c oxidase isoform expression in mouse embryos. Biochem Biophys. Acta 1260:361-364.

Grossman, L.I. 1995. Mitochondrial mutations and human disease. Environ. Mol. Mutagen 25:30-37.

Jaber, L.A., Slaughter, R.L., and Grunberger, G. 1995. Diabetes and related metabolic risk factors among Arab Americans. Ann. Pharmaco. Ther. 29:573-576.

Ko, M.S.H. 1995. Equalized cDNA libraries. In Reverse Transcriptase PCR (Larrick, J.W., and Siebert, P.D., Eds.) Simon and Schuster.

Kramer, J.A., and Krawetz, S.A. 1995 Matrix associated regions in haploid expressed domains. Mamm. Genome 6:677-679.

Krebs, C.J., McAvoy, M., and Kumar, G. 1995. JC virus minimal core promotion is glial specific in vivo. J. Virol. 69:2434-2442.

Kuivaniemi, H., Tromp, G., Bergfeld, W.F., Kay, M., and Helm, T.N. 1995. Ehlers-Danlos syndrome type IV: A single base substitution of the last nucleotide of exon 34 in COL3A1 leads to exon skipping. J. Invest. Dermat. 105:352-356.

Kuivaniemi, H., Tromp, G., and Prockop, D.J. 1996. Genetic causes of aortic aneurysms. In: Molecular Genetics and Gene Therapy of Cardiovascular Diseases. (Mockrin, S.C., ed.) Marcel Dekker, Inc., New York, pp.209-218.

Levy, J., Vandenberg, M., and Grunberger, G. 1995. Insulin versus glipizide treatment in patients with non-insulin-dependent diabetes mellitus. Effects on blood pressure and glucose tolerance. Am. J. Hypertens. 8:445-453.

Miller, O.J. 1995. The fifties and the renaissance in human and mammalian cytogenetics. Genetics 139:489-494.

Moshier, J.A., Malecka-Panas, E., Geng, H., Dosescu, J., Tureaud, J., Skunca, M., and Majumdar, A.P.N. 1995. Ornithine decarboxylase transformation of NIH/3T3 cells is mediated by altered epidermal growth factor receptor activity. Cancer Res. 55:5358-5365.

Nikolajczyk, B.S., Murray, M.T., and Hecht, N.B. 1995. A mouse homologue of the Xenopus germ cell-specific ribonucleic acid/deoxyribonucleic acid-binding proteins p54/p56 interacts with the protamine 2 promoter. Biol. Repr. 52:524-530.

Porter, C. A., Sampaio, I., Schneider, H., Czelusniak, J., and Goodman, M. 1995. Evidence on primate phylogeny from epsilon globin gene sequences and flanking regions. J. Mol. Evol. 40:30-55.

Quan, T.H., Reiners, J.J., Culp, S.J., Richter, P., and States, J.C. 1995. Differential mutagenicity and cytotoxicity of (+/-)-benzo(a)pyrene-trans-7,8- dihydrodiol and (+/-)-anti-benzo(a)pyrene-trans-7,8-dihydrodiol-9,10-epoxide in genetically engineered human fibroblasts. Mol. Carcinogenesis 12:91- 102.

Ronkainen, A., Hernesniemi, J., and Tromp, G. 1995. Special features of familial intracranial aneurysms: Report of 215 familial aneurysms. Neurosurgery 37:43-47.

Seelan, R.S., Gopalakrishnan, L., Scarpulla, R.C., and Grossman, L.I. 1996. Cytochrome c oxidase subunit VIIa liver isoform: Characterization and identification of promoter elements in the bovine gene. J. Biol. Chem. 271:2112-2120.

Singh, G.B., and Krawetz, S.A. 1995. CLONEPLACER: A software tool for simulating contig formation for OSS Ordered Shotgun Sequencing. Genomics 25:555-558.

Srinivas, P.R., Goustin, A.S., and Grunberger, G. 1995. Baculoviral expression of a natural inhibitor of the human insulin receptor tyrosine kinase. Biochim. Biophys. Res. Commun. 208:879-885.

Tomkiel, J., Fanti, L., Berloco, M., Spinelli, L., Tamkun, J.W., Wakimoto, B.T., and Pimpinelli, S. 1995. Developmental genetical analysis and molecular cloning of the abnormal oocyte gene of Drosophila melanogaster. Genetics 140:615-627.

Topping, R.S., Myrand, S.P., Williams, B.L., Albert, J.C., and States, J.C. 1995. Characterization of the human XPA promoter. Gene 166:341-342.

Tromp, G., De Paepe, A., Nuytinck, L., Madhatheri, S., and Kuivaniemi, H. 1995. Substitution of valine for glycine 793 in the triple-helical domain of type III procollagen in a mother and a child with the Ehlers-Danlos syndrome type IV. Hum. Mutat. 5:179-181.

Tugendreich, S., Tomkiel, J., Earnshaw, W.C., and Hieter, P. 1995. The CDC27Hs protein co-localizes with the CDC16Hs protein to the centrosome and mitotic spindle and is essential for the metaphase to anaphase transition. Cell 81:261-268.

Wykes, S.M., Nelson, J.E., Visscher, D.W., Djakiew, D., and Krawetz, S.A. 1995 Coordinate expression of the PRM1, PRM2 and TNP2 multigene locus in human testis. DNA Cell Biol. 14:155-161.

Zachar, V. , Goustin, A.S., Zacharova, V., Hager, H., Koppelhus, U., Womble, D.D., Liu, X., Bambra, C.S., Nyongo, A., and Ebbesen, P. 1996. Genetic polymorphism of envelope V3 region of HIV type 1 subtypes A, C, and D from Nairobi, Kenya. AIDS Res. Hum. Retroviruses 12:75-78.


The CMMG News is edited by Mary T. Murray, Ph.D. (313-577-4346, mmurray@cmb.biosci.wayne.edu), and David D. Womble, Ph.D. (313-577-2374, dwomble@cmb.biosci.wayne.edu).


Center for Molecular Medicine and Genetics
Wayne State University School of Medicine
3216 Scott Hall
540 East Canfield
Detroit, MI 48201
313-577-5323
cmmg@cmb.biosci.wayne.edu