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Restriction enzyme search

The restriction enzyme map function finds and displays restriction sites found within a specified region of a sequence. Users can select the enzyme types to search for.

[picture]
(Click for full size image)

This figure shows a typical view of the Restriction Enzyme Map function in which the results for most enzyme types are shown as black vertical lines opposite the enzyme names, but in which some of the enzymes sites have been configured by the user to be drawn in different colours. If no result is found for any particular enzyme eg here AccIII, the line will still be drawn so that zero cutters can be identified. The results can be scrolled vertically (and horizontally if the plot is zoomed in). A ruler is shown along the base and the current cursor (the vertical black line) position is shown in the left hand box near the top right of the display. If the user clicks, in turn, on two restriction sites their separation in base pairs will appear in the top right hand box. Information about the last site touched is shown in the Information line at the bottom of the display.


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This page is maintained by James Bonfield. Last generated on 2 Febuary 1999.
URL: http://www.mrc-lmb.cam.ac.uk/pubseq/manual/nip4_15.html