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Cloning Vector Clip

Filename
cloning_vector_clip.p4m

Description
This module searches for non "sequencing" vectors used in the shotgunning process, eg for Cosmid or YAC. Any fragment in any orientation of this vector could be present so there is no need for the cut sites to be known. The vector_clip program is used for this task (see section Screening against Vector Sequences).

Option: Vector file name
The filename containing the vector sequence. At present this should be a file containing a single plain text sequence containing just the bases or white space.

Option: Max probability
This option specifies the maximum probability of a particular match occurring by chance. Any match found with lower than this probability will be assumed to be real match, hence lower values represent more stringent matching.

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This page is maintained by James Bonfield. Last generated on 2 Febuary 1999.
URL: http://www.mrc-lmb.cam.ac.uk/pubseq/manual/pregap4_17.html