first previous next last contents

Estimate Base Accuracies

Filename
eba.p4m

Description
This module analyses the traces at each base call to estimate a confidence value for the called base. It does this by simply looking at the area underneath the trace for the called base and dividing this by the highest area under the trace for the three uncalled bases. This is a very simplistic statistic which should ideally only be used for measuring the average reliability of the entire sequence rather than any individual base. If another system (eg Phred) is available this should be used in preference. There are no adjustable parameters for this module.

first previous next last contents
This page is maintained by James Bonfield. Last generated on 2 Febuary 1999.
URL: http://www.mrc-lmb.cam.ac.uk/pubseq/manual/pregap4_8.html